Whole-cell amperometric biosensor for screening of cytochrome P450 inhibitors was written by Yoetz-Kopelman, Tal;Porat-Ophir, Carmit;Shacham-Diamand, Yosi;Freeman, Amihay. And the article was included in Sensors and Actuators, B: Chemical in 2016.Name: 1H-Benzo[d][1,2,3]triazol-1-amine This article mentions the following:
A fast and cost-effective whole-cell electrochem. biosensor aiming at early-stage screening of potential inhibitors of cytochrome P 450 is proposed and its feasibility demonstrated. Sensing is performed by monitoring the decrease in the electrochem. signal generated by the oxidation of the product of the enzymic reaction of cytochrome P 450 BM3 expressed in E. coli cells. The system is self-maintained and does not require enzyme purification or external addition of NADPH or other cofactors. Measurements were performed simultaneously at eight chips at low pos. potential of 100 mV vs. Ag/AgCl under continuous stirring. Kinetic anal. of aniline determination by the whole-cell system was performed and the concentration of aniline for the inhibition studies was selected accordingly. Three known inhibitors – imidazole, metyrapone and 1-aminobenzotriazole (ABT) – were tested and their inhibition profiles characterized. Imidazole was found to be the most potent inhibitor of the three. To the best of our knowledge, the system developed enables for the first time rapid and cheap cytochrome P 450 inhibitors detection by a disposable whole-cell electrochem. chip, combined with the advantages of a whole-cell system, without neither enzyme purification nor NADPH addition Furthermore, the system developed also provides capability of performing aniline detection e.g. for environmental monitoring, by means of electrochem. biosensing. In the experiment, the researchers used many compounds, for example, 1H-Benzo[d][1,2,3]triazol-1-amine (cas: 1614-12-6Name: 1H-Benzo[d][1,2,3]triazol-1-amine).
1H-Benzo[d][1,2,3]triazol-1-amine (cas: 1614-12-6) belongs to triazole derivatives. Triazoles exhibit substantial isomerism, depending on the positioning of the nitrogen atoms within the ring. Triazoles are compounds with a vast spectrum of applications, varying from materials (polymers), agricultural chemicals, pharmaceuticals, photoactive chemicals and dyes.Name: 1H-Benzo[d][1,2,3]triazol-1-amine
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics