An article The Effect of Light Intensity on the Expression of Leucoanthocyanidin Reductase in Grapevine Calluses and Analysis of Its Promoter Activity WOS:000585393400001 published article about PROANTHOCYANIDIN BIOSYNTHESIS; TRANSCRIPTION FACTOR; FLAVONOID BIOSYNTHESIS; FUNCTIONAL-CHARACTERIZATION; ANTHOCYANIN BIOSYNTHESIS; BERRY SKINS; GENE; PHENOLICS; ENZYMES; PATHWAY in [Cheng, Jing; Yu, Keji; Shi, Ying; Duan, Changqing; Wang, Jun] China Agr Univ, Coll Food Sci & Nutr Engn, Ctr Viticulture & Enol, Beijing 100083, Peoples R China; [Cheng, Jing; Yu, Keji; Shi, Ying; Duan, Changqing; Wang, Jun] Minist Agr & Rural Affairs, Key Lab Viticulture & Enol, Beijing 100083, Peoples R China; [Yu, Keji] Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China; [Zhang, Mingyue] Beijing Acad Food Sci, China Meat Res Ctr, Beijing 100068, Peoples R China in 2020.0, Cited 61.0. Recommanded Product: 61-82-5. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5
To investigate the effect of light intensity on flavonoid biosynthesis, grapevine calluses were subjected to high light (HL, 250 mu mol m(-2) s(-1)) and dark (0 mu mol m(-2) s(-1)) in comparison to 125 mu mol m(-2) s(-1) under controlled conditions (NL). The alteration of flavonoid profiles was determined and was integrated with RNA sequencing (RNA-seq)-based transcriptional changes of the flavonoid pathway genes. Results revealed that dark conditions inhibited flavonoid biosynthesis. Increasing light intensity affected flavonoids differently-the concentrations of flavonols and anthocyanins as well as the expressions of corresponding genes were less affected, whereas flavan-3-ol concentrations were predominantly increased, which caused enhanced trans-flavan-3-ol concentrations. Moreover, genes encoding leucoanthocyanidin reductase (LAR) exhibited different response patterns to light intensity changes-VviLAR1 expression increased with an increased light intensity, whereas VviLAR2 expression was insensitive. We further confirmed that the known transcription factors (TFs) involved in regulating flavan-3-ol biosynthesis utilized VviLAR1 as a target gene in grapevine calluses. In addition, VviLAR1 promoter activity was more sensitive to light intensity changes than that of VviLAR2 as determined using a transgenic Arabidopsis leaf system. These results suggested that light intensity had the most prominent effect on trans-flavan-3-ols in grapevine calluses and demonstrated that the two LAR genes had different response patterns to light intensity changes.
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Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
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