New learning discoveries about 1H-1,2,4-Triazol-5-amine

Welcome to talk about 61-82-5, If you have any questions, you can contact Kudyakova, YS; Onoprienko, AY; Slepukhin, PA; Burgart, YV; Saloutin, VI; Bazhin, DN or send Email.. COA of Formula: C2H4N4

COA of Formula: C2H4N4. In 2019.0 CHEM HETEROCYCL COM+ published article about ONE-POT SYNTHESIS; BUILDING-BLOCKS; HYDRAZINE; COMPLEXES; EFFICIENT; DERIVATIVES; LIGAND; YLIDE in [Kudyakova, Yulia S.; Slepukhin, Pavel A.; Burgart, Yanina V.; Saloutin, Victor I.; Bazhin, Denis N.] Russian Acad Sci, Ural Branch, Postovsky Inst Organ Synth, 22 S Kovalevskoi,20 Akad Skaya St, Ekaterinburg 620990, Russia; [Onoprienko, Aleksandra Ya.; Slepukhin, Pavel A.; Burgart, Yanina V.; Saloutin, Victor I.; Bazhin, Denis N.] Ural Fed Univ, 19 Mira St, Ekaterinburg 620002, Russia in 2019.0, Cited 53.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5.

Five- and six-membered fluorine-containing azaheterocycles were synthesized based on available furan-3(2H)-ones, and the influence of the nature of the fluoroalkyl substituent on the direction of the chemical transformations by the action of N,N- and N,O-binucleophiles was revealed.

Welcome to talk about 61-82-5, If you have any questions, you can contact Kudyakova, YS; Onoprienko, AY; Slepukhin, PA; Burgart, YV; Saloutin, VI; Bazhin, DN or send Email.. COA of Formula: C2H4N4

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Awesome and Easy Science Experiments about 61-82-5

Welcome to talk about 61-82-5, If you have any questions, you can contact Yu, L; Liu, WJ; Guo, ZW; Li, ZQ; Jiang, HY; Zou, Q; Mao, ZL; Fang, HC; Zhang, ZY; Wang, N; Chen, XS or send Email.. COA of Formula: C2H4N4

An article Interaction between MdMYB63 and MdERF106 enhances salt tolerance in apple by mediating Na+/H+ transport WOS:000581918000045 published article about R2R3-MYB TRANSCRIPTION FACTOR; R2R3-TYPE MYB GENE; STRESS TOLERANCE; EXPRESSION; REGULATOR; SALINITY; PATHWAY; FRUIT; SOS1 in [Yu, Lei; Liu, Wenjun; Guo, Zhangwen; Li, Zhiqiang; Jiang, Huiyan; Zou, Qi; Mao, Zuolin; Zhang, Zongying; Wang, Nan; Chen, Xuesen] Shandong Agr Univ, Coll Hort Sci, State Key Lab Crop Biol, 61 Daizong Rd, Tai An 271018, Shandong, Peoples R China; [Fang, Hongcheng] Shandong Agr Univ, Coll Forestry, State Forestry & Grassland Adm Key Lab Silvicultu, Tai An, Shandong, Peoples R China in 2020.0, Cited 34.0. COA of Formula: C2H4N4. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5

Salt stress is an important environmental factor affecting the growth and production of agricultural crops and fruits worldwide, including apple (Males x domestica). In this study, we demonstrate that a salt-responsive MYB transcription factor (TF), designated as MdMYB63, promotes survival under salt stress. Overexpression of MdMYB63 in apple calli significantly enhanced salt tolerance. Screening of the AP2/ERF family of TFs identified MdERF106 as an interaction partner of MdMYB63. Further analyses showed that the MdMYB63-MdERF106 complex significantly promotes the expression of downstream MdSOS1, thereby improving the Na+ expulsion and salt tolerance of apple. These functional analyses of MdMYB63 have provided valuable insights into the regulatory network of salt tolerance, and lay a theoretical foundation for the cultivation of new salt-tolerant apple varieties.

Welcome to talk about 61-82-5, If you have any questions, you can contact Yu, L; Liu, WJ; Guo, ZW; Li, ZQ; Jiang, HY; Zou, Q; Mao, ZL; Fang, HC; Zhang, ZY; Wang, N; Chen, XS or send Email.. COA of Formula: C2H4N4

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

More research is needed about 1H-1,2,4-Triazol-5-amine

SDS of cas: 61-82-5. Welcome to talk about 61-82-5, If you have any questions, you can contact Aumeier, BM; Dang, AHQ; Ohs, B; Yuce, S; Wessling, M or send Email.

Aumeier, BM; Dang, AHQ; Ohs, B; Yuce, S; Wessling, M in [Aumeier, Benedikt M.; Dang, Anh H. Q.; Ohs, Burkhard; Yuece, Sueleyman; Wessling, Matthias] Rhein Westfal TH Aachen, Aachener Verfahrenstech, Chem Proc Engn, Forckenbeckstr 51, D-52074 Aachen, Germany; [Wessling, Matthias] DWI Leibniz Inst Interact Mat, Forckenbeckstr 50, D-52074 Aachen, Germany published Aqueous-Phase Temperature Swing Adsorption for Pesticide Removal in 2019.0, Cited 67.0. SDS of cas: 61-82-5. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5.

Recently, activated carbon adsorption for water treatment regained substantial attention due to the emerging task to remove trace organic compounds such as pesticides. In many applications, especially in decentralized water treatment, one major drawback of adsorbents is their limited recyclability due to inadequate logistics or uneconomical reactivation. In this lab-scale study, we present the temperature swing adsorption in the aqueous phase that allows the in situ regeneration of fixed-bed adsorbers, and prove its technical feasibility. Complying with circular water economy principles, we eliminated the pivotal need for regular replacement and consumables by employing only clean water instead of dedicated regeneration solutions. Adsorption of the herbicide amitrole in aqueous solution on granular activated carbon was exothermic (Delta H = -14.4 +/- 3.2 kJ mol(-1) for T = 20-94 degrees C) and followed the Freundlich model. The proposed method consisting of a short counterflow flush with liquid water at 125 degrees C effectively regenerated the adsorbent. Hence, we obtained a cyclic steady state operation with breakthrough after 122 +/- 14 bed volumes (at c(out)/c(in) = 0.2), cycle-average rejection of 90 +/- 1%, and water recovery of up to 78 +/- 4%. No thermal aging of adsorbent was observed over the investigated 17 cycles.

SDS of cas: 61-82-5. Welcome to talk about 61-82-5, If you have any questions, you can contact Aumeier, BM; Dang, AHQ; Ohs, B; Yuce, S; Wessling, M or send Email.

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

The important role of 61-82-5

Welcome to talk about 61-82-5, If you have any questions, you can contact Zhu, TY; Yu, X; Yi, M; Wang, Y or send Email.. Product Details of 61-82-5

I found the field of Chemistry; Science & Technology – Other Topics; Engineering very interesting. Saw the article Facile Covalent Crosslinking of Zeolitic Imidazolate Framework/Polydimethylsiloxane Mixed Matrix Membrane for Enhanced Ethanol/Water Separation Performance published in 2020.0. Product Details of 61-82-5, Reprint Addresses Wang, Y (corresponding author), Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Minist Educ, Key Lab Mat Chem Energy Convers & Storage, Wuhan 430074, Peoples R China.; Wang, Y (corresponding author), Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Hubei Key Lab Mat Chem & Serv Failure, Wuhan 430074, Peoples R China.. The CAS is 61-82-5. Through research, I have a further understanding and discovery of 1H-1,2,4-Triazol-5-amine

The interfacial design is critical in preparing high-performance mixed matrix membranes (MMMs), especially for the separation of larger sized components from mixtures. Herein, a facile and novel strategy was employed to design covalently linked zeolitic imidazolate framework-8-polydimethylsiloxane (ZIF-8@PDMS) MMMs without interfacial defects by a onestep synthesis route for ethanol recovery from ethanol aqueous solution. In this strategy, 3-glycidyloxypropyltrimethoxysilane (GOPTS) worked as the covalent linker of PDMS and amine-functionalized ZIF-8 nanoparticles (AZIF-8) simultaneously. The chemical structures and morphologies of AZIF-8 and AZIF-8@PDMS MMMs were demonstrated by various characterization techniques. The results exhibited that AZIF-8 and the MMMs were successfully prepared and AZIF-8 as the filler displayed better dispersion in the PDMS matrix and compatibility with the PDMS matrix as compared to ZIF-8 or GOPTS-modified ZIF-8 (GZIF-8). Therefore, AZIF-8@PDMS MMMs showed more excellent separation performance than ZIF-8 or GZIF-8-filled MMMs. In particular, AZIF-8@PDMS MMM with 7 wt % AZIF-8 loading exhibited the highest separation factor of 17.7 and a comparable total flux of 585.6 g/m(2) h at 40 degrees C with 5 wt % ethanol aqueous solution, which were improved by 176.6 and 34.5%, respectively, in comparison with the pristine PDMS membrane, breaking the trade-off effect between the flux and separation factor. This study might provide some new insights into the fabrication of high-performance MMMs for pervaporation recovery of various organic systems.

Welcome to talk about 61-82-5, If you have any questions, you can contact Zhu, TY; Yu, X; Yi, M; Wang, Y or send Email.. Product Details of 61-82-5

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

What Kind of Chemistry Facts Are We Going to Learn About 1H-1,2,4-Triazol-5-amine

Safety of 1H-1,2,4-Triazol-5-amine. Welcome to talk about 61-82-5, If you have any questions, you can contact Liu, SY; Huang, XH; Hu, KJ; Jin, Q; Zhu, GN or send Email.

Safety of 1H-1,2,4-Triazol-5-amine. In 2020.0 J CHROMATOGR SCI published article about TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMAN HEALTH-RISK; ORGANOCHLORINE PESTICIDES; TRACE DETERMINATION; POLAR PESTICIDES; HPLC-DAD; PHARMACEUTICALS; RIVER; SPE in [Liu, Shaoying; Huang, Xihui; Hu, Kejun; Jin, Quan] Hangzhou Ctr Dis Control & Prevent, Lab Chem & Phys, Hangzhou, Peoples R China; [Zhu, Guonian] Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou, Peoples R China in 2020.0, Cited 44.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5.

A rapid and efficient method based on solid phase extraction and liquid chromatography-tandem mass spectrometry was validated, allowing the determination of the endocrine-disrupting herbicides (acetochlor, alachlor, amitrole and atrazine), fungicides (carbendazim, triadimefon, penconazole and propiconazole), and insecticides (carbaryl and carbofuran) in drinking water. Low method detection limits (0.01-0.64 ng/L) and method quantification limits (0.03-2.13 ng/L) were obtained with satisfactory recoveries and precision for the endocrine-disrupting pesticides. The method was applied for real drinking water samples collected in the area of the city of Hangzhou (Zhejiang, China); the results showed that carbendazim, atrazine and acetochlor were detected in the drinking water samples and acetochlor was the most detected analyte.

Safety of 1H-1,2,4-Triazol-5-amine. Welcome to talk about 61-82-5, If you have any questions, you can contact Liu, SY; Huang, XH; Hu, KJ; Jin, Q; Zhu, GN or send Email.

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

New explortion of C2H4N4

Bye, fridends, I hope you can learn more about C2H4N4, If you have any questions, you can browse other blog as well. See you lster.. HPLC of Formula: C2H4N4

HPLC of Formula: C2H4N4. In 2020.0 EUR J MED CHEM published article about BIOLOGICAL EVALUATION; AUTOPHAGY; APOPTOSIS; ROS; DESIGN in [Wang, Shuai; Ma, Xu-Bin; Yuan, Xiao-Han; Yu, Bin; Xu, Yi-Chao; Liu, Hong-Min] Zhengzhou Univ, Sch Pharmaceut Sci, Zhengzhou 450001, Peoples R China; [Wang, Shuai; Ma, Xu-Bin; Yuan, Xiao-Han; Yu, Bin; Xu, Yi-Chao; Liu, Hong-Min] Zhengzhou Univ, Key Lab Adv Drug Preparat Technol, Mil Educ, Zhengzhou 450001, Peoples R China; [Wang, Shuai] Massachusetts Gen Hosp, Gordon Ctr Med Imaging, Boston, MA 02129 USA; [Wang, Shuai] Harvard Med Sch, Boston, MA 02129 USA in 2020.0, Cited 33.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5.

Mitochondria are known as powerhouse of cells and play the role of a bridge in redox balance, cell apoptosis, and autophagy. ROS accumulation can cause mitochondria damage, while the injured mitochondria will further enhance ROS levels reciprocally. Herein, we synthesized a novel series of [1,2,4] triazolo[1,5-a]pyrimidine-based compounds 4a-4v and tested their anti-proliferation efficacy against gastric cancer cell line MGC-803. Among them, compounds 4o and 4p inhibited gastric cancer cells at micromolar level. Compound 4o caused G2/M arrest and induced mitochondria-dependent apoptosis in MGC-803 and SGC-7901. However, inhibiting apoptosis pathway cannot prevent the inhibitory activity of compound 4o against gastric cancer cell. To our surprising, ROS level was increased by compound 4o and elevation of ROS could be rescued by NAC. In accordance with that, NAC absolutely prevented the anti-proliferation efficacy of compound 4o. We further found that autophagy inhibitor CQ rather than 3-MA partially reversed inhibitory activity of compound 4o in MGC-803 cells. Taken together, compound 4o exhibited its anti-proliferative activity via increasing ROS level and inducing autophagy, thus leading to apoptosis of gastric cancer cells. Therefore, compound 4o may support further development of lead compounds for gastric cancer therapy via mitochondria pathway. (C) 2020 Elsevier Masson SAS. All rights reserved.

Bye, fridends, I hope you can learn more about C2H4N4, If you have any questions, you can browse other blog as well. See you lster.. HPLC of Formula: C2H4N4

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Archives for Chemistry Experiments of 61-82-5

Welcome to talk about 61-82-5, If you have any questions, you can contact Li, JJ; Zhou, YN; Luo, ZH; Zhu, SP or send Email.. SDS of cas: 61-82-5

I found the field of Polymer Science very interesting. Saw the article A polyelectrolyte-containing copolymer with a gas-switchable lower critical solution temperature-type phase transition published in 2019.0. SDS of cas: 61-82-5, Reprint Addresses Zhu, SP (corresponding author), McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L7, Canada.; Luo, ZH (corresponding author), Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Dept Chem Engn, Shanghai 200240, Peoples R China.; Zhu, SP (corresponding author), Chinese Univ Hong Kong, Sch Sci & Engn, Shenzhen 518172, Peoples R China.. The CAS is 61-82-5. Through research, I have a further understanding and discovery of 1H-1,2,4-Triazol-5-amine

A thermo-responsive copolymer with a gas-switchable LCST-type phase transition was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization of N-isopropylacrylamide (NIPAM) and glycidyl methacrylate (GMA), followed by post-polymerization functionalization with sodium 3-amino-1,2,4-triazole (ATANa). Incorporating ionic moieties provides an elevated cloud point of 61 degrees C. Importantly, both CO2 and SO2 cause a reduction in the cloud point of the polymer solution. The CO2-triggered system can be easily and fully recovered to its initial state by introducing an inert gas (e.g. N-2), whereas the SO2-triggered system shows only partial recovery. The pH-dependent phase transition behaviors confirm that the gas bubbling-induced pH changes contribute to the gas-switchable cloud point of P(NIPAM-co-(GMA-ATANa)). In addition, P(NIPAM-co-(GMA-ATA)) (ATA: 3-amino-1,2,4-triazole), a counterpart of P(NIPAM-co-(GMA-ATANa)), was prepared and the relevant solution phase transition behavior was studied. Its cloud point shift in response to gas bubbling and pH adjustment is opposite to that of P(NIPAM-co-(GMA-ATANa)), indicating that the latter does not result from the protonation of amidine groups. Alternatively, a reversible H+-induced decrease in hydrophilicity was thus proposed. This contribution enriches the family of thermo-responsive polymers by introducing gas-sensitive polyelectrolytes and also broadens the scope of gas-responsive smart materials.

Welcome to talk about 61-82-5, If you have any questions, you can contact Li, JJ; Zhou, YN; Luo, ZH; Zhu, SP or send Email.. SDS of cas: 61-82-5

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

New explortion of 1H-1,2,4-Triazol-5-amine

Bye, fridends, I hope you can learn more about C2H4N4, If you have any questions, you can browse other blog as well. See you lster.. Product Details of 61-82-5

I found the field of Biochemistry & Molecular Biology; Chemistry very interesting. Saw the article The Role of Micropores and Amino Groups in Preferential CO2 Adsorptivity of Porous Zn-Coordination Polymers Comprising Mixed Ligands of Triazole and Amino Triazole published in 2019.0. Product Details of 61-82-5, Reprint Addresses Zubir, M (corresponding author), State Univ Medan, Fac Math & Nat Sci, Chem Dept, Jl Willem Iskandar,Pasar 5,Medan Estate, Medan 20221, North Sumatera, Indonesia.. The CAS is 61-82-5. Through research, I have a further understanding and discovery of 1H-1,2,4-Triazol-5-amine

Porous coordination polymers (PCPs) of Zn2+, oxalic acid and mixture ratio of 3-amino, 1,2,4-triazole (Ataz) and 1,2,4-triazole (Taz) were synthesized to capture of carbon dioxide. A series of molar fraction Taz/(Taz + ATaz) prepared as 0.1;0.3;0.4;0.5;0.6;0.7 and 0.9 and also only ATaz and Taz ligand as 0 and 1 molar fraction respectively. Mixture of Taz and ATaz crystal induce new structure of 0.4, 0.5 and 0.6 molar fraction. Nitrogen adsorption of 50% mixture each ligand contribute the highest surface area as function of optimum condition to integrate pore space and amine group presence with adsorbed as 290 mgg(-1). This phenomena also shown through CO2 adsorption amount as 135 mgg(-1), instead of 0.4, 0.6 and 0.7 molar fraction observed higher amount of CO2 compared with only Taz ligand (X=1), indicate the integrated effects both of the ligand could generated the capture of higher amount of carbon dioxide.

Bye, fridends, I hope you can learn more about C2H4N4, If you have any questions, you can browse other blog as well. See you lster.. Product Details of 61-82-5

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Extended knowledge of C2H4N4

Computed Properties of C2H4N4. Bye, fridends, I hope you can learn more about C2H4N4, If you have any questions, you can browse other blog as well. See you lster.

An article A new theory of two-step stabilization mechanism for triazole-based zinc-containing complex as thermal stabilizer for polyvinyl chloride) WOS:000488663100010 published article about POLY(VINYL CHLORIDE); HEAT STABILIZERS; PENTAERYTHRITOL; INHIBITION; EFFICIENT; FUSION in [Wang, Xu; Ma, Biao; Wang, Yating; Lu, Songyan; Ma, Meng; Shi, Yanqin; Chen, Si] Zhejiang Univ Technol, Coll Mat Sci & Engn, Hangzhou 310014, Zhejiang, Peoples R China in 2019.0, Cited 31.0. Computed Properties of C2H4N4. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5

A totally new theory of two-step stabilization mechanism for triazole-based zinc-containing complex (abbreviated as (Zn(ttr)(OAc)]) was proposed through several designed experiments. [Zn(ttr)(OAc)] strongly absorbed HCl at the beginning, and then broke down into 3-amino-1,2,4-triazole which could further stabilized PVC. The structure of [Zn(ttr)(OAc)] was characterized by FTIR spectroscopy, elemental analysis and SEM. Then, Congo red tests and discoloration tests verified [Zn(ttr)(OAc)] could absorb HCl effectively, but PVC showed poor initial whiteness, which further proved above -mentioned stabilization mechanism. Moreover, [Zn(ttr)(OAc)] combined with ZnSt(2) and dibenzoyl methane (DBM) effectively improved the initial color of PVC samples and provided PVC with shorter plasticizing time and longer dynamic thermal stability time than commercial stabilizer 1086F, confirmed by discoloration tests and dynamic thermal stability analysis. We believe [Zn(ttr)(OAc)]/ZnSt(2)/DBM stabilizer system can be used as a commercial stabilizer and the stabilization mechanism of [Zn(ttr)(OAc)] may provide a new way to stabilize PVC. (C) 2019 Published by Elsevier Ltd.

Computed Properties of C2H4N4. Bye, fridends, I hope you can learn more about C2H4N4, If you have any questions, you can browse other blog as well. See you lster.

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Final Thoughts on Chemistry for C2H4N4

Safety of 1H-1,2,4-Triazol-5-amine. Welcome to talk about 61-82-5, If you have any questions, you can contact Andrews, SS; Schaefer-Ramadan, S; Al-Thani, NM; Ahmed, I; Mohamoud, YA; Malek, JA or send Email.

An article High-resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-Seq) WOS:000478717600017 published article about P53 TUMOR-SUPPRESSOR; CRYSTAL-STRUCTURE; COMPLEXES; SYSTEM in [Andrews, Simeon S.; Schaefer-Ramadan, Stephanie; Al-Thani, Nayra M.; Ahmed, Ikhlak; Malek, Joel A.] Weill Cornell Med Qatar, Dept Genet Med, Doha 24144, Qatar; [Mohamoud, Yasmin A.; Malek, Joel A.] Weill Cornell Med Qatar, Genom Lab, Doha 24144, Qatar in 2019.0, Cited 18.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5. Safety of 1H-1,2,4-Triazol-5-amine

Two-hybrid systems can be used for investigating protein-protein interactions and may provide important information about gene products with unknown function. Despite their success in mapping protein interactions, two-hybrid systems have remained mostly untouched by improvements in next-generation DNA sequencing. The two-hybrid systems rely on one-versus-all methods in which each bait is sequentially screened against an entire library. Here, we developed a screening method that joins both bait and prey as a convergent fusion into one bacterial plasmid vector that can then be amplified and paired-end sequencing by next-generation sequencing (NGS). Our method enables all-versus-all sequencing (AVA-Seq) and utilizes NGS to remove multiple bottlenecks of the two-hybrid system. AVA-Seq allows for high-resolution protein-protein interaction mapping of a small set of proteins and has the potential for lower-resolution mapping of entire proteomes. Features of the system include ORF selection to improve efficiency, high bacterial transformation efficiency, a convergent fusion vector to allow paired-end sequencing of interactors, and the use of protein fragments rather than full-length proteins to better resolve specific protein contact points. We demonstrate the system’s strengths and limitations on a set of proteins known to interact in humans and provide a framework for future large-scale projects.

Safety of 1H-1,2,4-Triazol-5-amine. Welcome to talk about 61-82-5, If you have any questions, you can contact Andrews, SS; Schaefer-Ramadan, S; Al-Thani, NM; Ahmed, I; Mohamoud, YA; Malek, JA or send Email.

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics