In 2015,Jonker, Anika M.; Bode, Saskia A.; Kusters, Addie H.; van Hest, Jan C. M.; Loewik, Dennis W. P. M. published 《Soft PEG-Hydrogels with Independently Tunable Stiffness and RGDS-Content for Cell Adhesion Studies》.Macromolecular Bioscience published the findings.Category: triazoles The information in the text is summarized as follows:
Poly(ethylene)glycol (PEG)-based hydrogels are often used as matrix material for cell culturing. An efficient method to prepare soft PEG gels is by crosslinking via copper-free strain-promoted azide-alkyne cycloaddition (SPAAC). Here, the effect of polymer d. and RGDS-content on hydrogel formation and cell adhesion was studied, by varying the total polymer content (10, 20 and 30 mg · mL-1) and the amount of RGDS moieties (0-100%) independently of each other. Rheol. studies confirmed the soft nature of the hydrogels (G’ = 25-2 298 Pa). HOS cells are able to adhere well to all RGDS-containing gels. Interestingly, both HeLa cells and NIH 3T3 fibroblasts showed substantial adherence to 10 and 20 mg · mL-1 gels, but with increased hydrogel stiffness (30 mg · mL-1), their cellular adhesion decreased significantly. In the part of experimental materials, we found many familiar compounds, such as ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)(cas: 56602-33-6Category: triazoles)
((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)(cas: 56602-33-6) is used as a reagent for peptide coupling, lactonization, selective esterification, amidation of alfa amino acids without racemization and synthesis of magnolamide for antioxidative activity and catalyst for 9-acridinecaroboxamide derivative. It is also used as a precursor for the synthesis of phenyl esters of amino acids.Category: triazoles
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics