In 2018,Bojtar, Marton; Janzso-Berend, Peter Zoltan; Mester, David; Hessz, Dora; Kallay, Mihaly; Kubinyi, Miklos; Bitter, Istvan published 《An uracil-linked hydroxyflavone probe for the recognition of ATP》.Beilstein Journal of Organic Chemistry published the findings.Synthetic Route of C30H30N10 The information in the text is summarized as follows:
Nucleotides are essential mols. in living systems due to their paramount importance in various physiol. processes. In the past years, numerous attempts were made to selectively recognize and detect these analytes, especially ATP using small-mol. fluorescent chemosensors. Despite the various solutions, the selective detection of ATP is still challenging due to the structural similarity of various nucleotides. In this paper, we report the conjugation of a uracil nucleobase to the known 4′-dimethylamino-hydroxyflavone fluorophore. The complexation of this scaffold with ATP is already known. The complex is held together by stacking and electrostatic interactions. To achieve multi-point recognition, we designed the uracil-appended version of this probe to include complementary base-pairing interactions. The theor. calculations revealed the availability of multiple complex structures. The synthesis was performed using click chem. and the nucleotide recognition properties of the probe were evaluated using fluorescence spectroscopy. The first, uracil-containing fluorescent ATP probe based on a hydroxyflavone fluorophore was synthesized and evaluated. A selective complexation with ATP was observed and a ratiometric response in the excitation spectrum. In the experiment, the researchers used many compounds, for example, Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8Synthetic Route of C30H30N10)
Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8) can stabilizes Cu(I) towards disproportionation and oxidation thus enhancing its catalytic effect in the azide-acetylene cycloaddition.Synthetic Route of C30H30N10
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics