In 2015,Neuner, Sandro; Santner, Tobias; Kreutz, Christoph; Micura, Ronald published 《The “”Speedy”” Synthesis of Atom-Specific 15N Imino/Amido-Labeled RNA》.Chemistry – A European Journal published the findings.Electric Literature of C12H22F6N6OP2 The information in the text is summarized as follows:
Although numerous reports on the synthesis of atom-specific 15N-labeled nucleosides exist, fast and facile access to the corresponding phosphoramidites for RNA solid-phase synthesis is still lacking. This situation represents a severe bottle-neck for NMR spectroscopic investigations on functional RNAs. Here, we present optimized procedures to speed up the synthesis of 15N(1)adenosine and 15N(1)guanosine amidites, which are the much needed counterparts of the more straightforward-to-achieve 15N(3) uridine and 15N(3) cytidine amidites in order to tap full potential of 1H/15N/15N-COSY experiments for directly monitoring individual Watson-Crick base pairs in RNA. Demonstrated for two preQ1 ribo-switch systems, we exemplify a versatile concept for individual base-pair labeling in the anal. of conformationally flexible RNAs when competing structures and conformational dynamics are encountered. The results came from multiple reactions, including the reaction of ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)(cas: 56602-33-6Electric Literature of C12H22F6N6OP2)
((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)(cas: 56602-33-6) is a peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.Electric Literature of C12H22F6N6OP2
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics